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. 2006 Sep 13;34(17):4780–4790. doi: 10.1093/nar/gkl631

Figure 1.

Figure 1

Expression of ZFP206 in ES cells. (A) Forty-three known and predicted genes expressed with high specificity in ES cells were identified by sorting microarray profiles (16) to identify genes whose expression in ES cells is higher than in 54 other tissues examined, then clustering to remove those that are also appreciably expressed in embryos or other tissue sets. In some cases, multiple loci encode very similar proteins, which are represented here by a single cDNA Accession number. ‘*’ indicates genes that are not found in the MGI database and therefore have no standard name. (B) Real time PCR on 38 mouse tissues and ES cells confirming that ZFP206 is primarily expressed in R1 ES cells. (C) Northern blot analysis of ZFP206 expression in three pluripotent ES cell lines (R1, D3 and E14TG2a) co-cultured with MEF. (D) Subcellular localization of 3XFLAG tagged Zfp206 cDNA (isoform 1) in R1 ES cells. (E) Western blotting of 3XFLAG tagged Zfp206 cDNA (isoform 1) in R1 ES cells. The expected MW of the tagged protein is ∼90 kDa.