Figure 5.

Effect of ZFP206 expression on ES cell differentiation. (A) Parental ES cells (E14TG2a for overexpression clones and R1 for knockdown clones), three ZFP206 overexpression, one knockdown, and corresponding empty vector control cell lines were analyzed following plating at clonal density (see Materials and Methods) using normal ES cell medium with LIF. After 6 days of culture, the percentage of pure alkaline phosphatase positive colonies were calculated. Data are the mean and SDs of three repeats. (B) Examples of uniformly undifferentiated and differentiated clones.