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. 2006 Sep 18;34(17):4976–4986. doi: 10.1093/nar/gkl660

Figure 3.

Figure 3

Cellular localization of mutant XE7. (A) XE7 pre-mRNA. The exons are shown as yellow boxes and introns as horizontal lines. The blue and green lines connecting the exons indicate the splicing patterns, either including or omitting exon 5. The blue lines depict inclusion of the alternative exon (exon 5), resulting in a premature stop codon and leading to production of the short isoform of XE7. The green lines show production of the long isoform. The start and stop codons are marked with arrows. (B) Full-length XE7 is localized to nuclear speckles in HeLa cells. A schematic representation of the full-length construct used is shown on the left. (C) Deletion of the RS-rich region causes XE7 to localize to the perinuclear region of the cytoplasm. XE7ΔRS indicates the XE7 form with the RS-rich region deleted, shown on the left. Scale bars: 10 μm.