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. 2006 Sep 18;34(17):4976–4986. doi: 10.1093/nar/gkl660

Figure 4.

Figure 4

Interaction of XE7 with ZNF265 and ASF/SF2 in yeast two-hybrid assays. Yeast were cotransformed with DNA-binding-plasmids (vector pGBK = bait) and activation-plasmids (vector pGAD or pACT =prey) encoding the proteins indicated, and colonies were grown on SD (−A/−H/−L/−W) dropout plates prior to β-galactosidase assay. p53 and T-antigen interaction was used as a positive control, and T-antigen and LamC as negative control. ZNF265SF = ZNF265 short form, ZNF265LF = ZNF265 long form. XE7ΔRS = XE7 with the RS-rich region deleted.