Figure 4.

dmExp5 binds tRNA more preferentially than hsExp5. Upper panel: Bacterially expressed dmExp5 (lanes 1–9) or hsExp5 (lanes 10–14) were mixed with radio-labeled pre-miR-30 and RanQ69L-GTP orthologues from cognate species in the absence (lanes 1 and 10) or presence of 5 pmol (lanes 2, 6 and 11), 10 pmol (lanes 3, 7 and 12), 25 pmol (lanes 4, 8 and 13) or 50 pmol (lanes 5, 9 and 14) of indicated competitor RNAs. Protein–RNA complexes were detected by EMSA. Arrows indicate the positions of bound and free probe. Lower panel: Binding efficiency was calculated as in Figure 2B. Data are represented by means + SD of three independent experiments. Note that the concentrations of competitor RNAs were 10 times higher than those in Figure 2.