Figure 6.

The repression of miRNA and tRNA levels induced by knockdown of dmExp5 was restored by overexpression of hsExp5. S2/hsExp5, a S2 cell derivative stably expressing hsExp5, was treated with 111 nM of the indicated dsRNAs as described in Figure 6B. S2/Emp, which harbors an empty vector, was used as a negative control. CuSO₄ was added to the culture media 24 h after dsRNA treatment (see Materials and Methods). (A) Depletion of dmExp5 was confirmed by RT–PCR (upper and middle panels). Expression of hsExp5 was confirmed by western blot using anti-FLAG M2 antibody (lower panel). (B) Northern blot analysis was performed as described in Figure 6A. The repression of miRNA and tRNA induced by knockdown of dmExp5 was partially restored by the expression of hsExp5.