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. 2006 Nov 26;34(20):5951–5965. doi: 10.1093/nar/gkl689

Figure 1.

Figure 1

Dlx2 binds to an element in the Msx2 Promoter. (A) Dlx2 proteins (60, 120 and 180 ngs) were incubated with the Msx2 promoter sequence containing a Dlx2 binding element (TAGTTG) as the radioactive probe. The 50× comp. lane was performed with 180 ngs of Dlx2 protein and 50-fold excess of cold competitor probe. (B) Dlx2 protein (180 ng) was incubated with 2 μl of Dlx2 Ab, which decreased Dlx2 binding demonstrating the specificity of Dlx2. Msx2 protein at 60, 120 and 180 ng bound the Msx2 probe as three distinct bands. Msx2 titration (120 and 180 ng) with 180 ng Dlx2 revealed competitive binding for the same element by these proteins. The EMSA experiments were analyzed in 8% native polyacrylamide gels. The free and bound forms of DNA were quantitated using the Molecular Dynamics STORM PhosphoImager. The free probe, bound and dimer complexes are indicated.