Figure 10.

Msx2 auto-regulation and repression of Dlx2 activation. (A) CHO cells were transfected with either the Msx2-5820 luciferase reporter gene or the Msx2-872 luciferase reporter gene. The cells were co-transfected with CMV-Dlx2 and/or CMV-Msx2 or the CMV empty vector. The concentrations of the reporter plasmids were 5 μg and 2.5 μg for the expression plasmids. (B) C3H10T1/2 cells were transfected as in (A) to determine if the activation was cell dependent. All transfection assays were performed as described in Figure 5. The activities are shown relative to Msx2 promoters without Dlx2 and Msx2 expression (+/− SEM from five independent experiments).