Figure 11.

Msx2 expression attenuates Dlx2 and Lef-1 synergistic activation of the Msx2 promoter. (A) CHO cells were co-transfected with the Msx2-5820 luciferase reporter gene, CMV Dlx2, CMV Lef-1 FL or CMV Lef-1 ΔN113. The CMV Msx2 expression plasmid was titrated from 1.25 to 3.75 μg in the indicated experiments. The concentration of the reporter plasmid was 5 μg and Dlx2 and Lef-1 expression plasmids were 2.5 μg. (B) LS-8 cells were transfected as in (A) to demonstrate the activation of the Msx2 promoter in these tooth epithelial cells. All transfection assays were performed as described in Figure 5. The activities are shown relative to Msx2 promoters without Dlx2, Lef-1 and Msx2 expression (+/− SEM from four independent experiments).