Figure 3.

Dlx2 binds to the Msx2 promoter in vivo. (A) Schematic of the 5.8 Kb Msx2 promoter with the Dlx2 binding sites noted by asterisks. The location of the sense primer (S) and the anti-sense primer (AS) are shown in the blowup of the −555 to −13 bp region of the proximal promoter used to amplify the immunoprecipiated chromatin. (B) ChIP assays were performed using CHO cells. Lane 1 contains the 1 Kb ladder and lane 2 is the Dlx2 immunoprecipitated chromatin amplified using the specific Msx2 promoter primers and produced the correct size product of 272 bp. Lane 3 is Msx2 primers only; lane 4 is the chromatin input using the Msx2 promoter primers. Lane 5 is the immunoprecipitation using normal rabbit IgG and Msx2 primers and lane 6 is the Dlx2 immunoprecipitated chromatin amplified with primers to an unrelated gene. (C) ChIP assays were performed using LS-8 cells and the same experimental procedures as in panel B. Experiments were repeated three separate times.