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. 2006 Nov 26;34(20):5951–5965. doi: 10.1093/nar/gkl689

Figure 4.

Figure 4

Lef-1 binds to the Msx2 promoter in vivo. (A) Schematic of the 5.8 Kb Msx2 promoter with the Lef-1 binding site (CTTTGTC) shown in the proximal promoter. The location of the sense primer (S) and the anti-sense primer (AS) are shown in the blowup of the −555 to −13 bp region of the proximal promoter used to amplify the immunoprecipitated chromatin. (B) ChIP assays were performed using LS-8 cells. Lane 1 contains the 1 Kb ladder and lane 2 is the Lef-1 immunoprecipitated chromatin amplified using the specific Msx2 promoter primers and produced the correct size product of 272 bp. Lane 3 is Msx2 primers only; lane 4 is the chromatin input using the Msx2 promoter primers. Lane 5 is the immunoprecipitation using normal rabbit IgG and Msx2 primers and lane 6 is the Lef-1 immunoprecipitated chromatin amplified with primers to an unrelated gene.