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. 2006 Nov 26;34(20):5951–5965. doi: 10.1093/nar/gkl689

Figure 8.

Figure 8

Identification of Lef-1 domains required for synergism with Dlx2. (A) A schematic of the Lef-1 deletion constructs used in the transfection assays. βBD, β-catenin binding domain; CAD, context-dependent activation domain; HMG, high mobility group domain. The numbers under the constructs denote residues. (B) CHO cells were transfected with the Msx2-5820 luciferase reporter gene (5 μg). The cells were co-transfected with the CMV-Dlx2 and/or CMV-Lef-1 full-length (FL), Lef-1 short isoform (Lef-1 ΔN113), the Lef-1 deletion construct expression plasmids, the CMV plasmid without Dlx2 or Lef-1 (−) (2.5 μg). (C) C3H10T1/2 cells were transfected as in (B). To control for transfection efficiency, all transfections included the SV-40 β-galactosidase reporter (0.5 μg). Cells were incubated for 24 h and then assayed for luciferase and β-galactosidase activities. The activities are shown as mean fold activation compared to the Msx2 promoter plasmid without Dlx2 or Lef-1 expression and normalized to β-galactosidase activity (+/− SEM from five independent experiments).