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. 2006 Nov 26;34(20):5951–5965. doi: 10.1093/nar/gkl689

Figure 9.

Figure 9

Dlx2 binds to two regions of the Lef-1 protein. (A) Schematic of the Lef-1 deletion proteins used in the GST-pull-down assays. The location of the two Dlx2 interaction regions of Lef-1 are shown and bracketed by lines and designated Dlx2 binding domain (BD) #1 and 2. (B) GST-Lef-1 and truncated Lef-1 protein pull-down assay with bacterial expressed and purified Dlx2 protein (150 ng). To demonstrate Dlx2 binding to Lef-1, independent of the βBD, the Lef-1 short isoform (GST-Lef-1ΔN113) was incubated with pure Dlx2 protein. Dlx2 binds to the Lef-1 short isoform as well as the last 34 residues of the Lef-1 C-terminal tail (GST-Lef-1ΔN363). This region is termed Dlx2 BD #2. (C) Dlx2 binding to the Lef-1 C-terminal truncation mutants. Dlx2 binds to a region containing the CAD and 3′ flanking residues and termed Dlx2 BD #1. As a control GST-beads were incubated with purified Dlx2 to demonstrate the specificity of Dlx2 binding to the GST-Lef-1 fusion proteins.