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. 2006 Oct 4;34(19):5552–5566. doi: 10.1093/nar/gkl691

Figure 4.

Figure 4

Determination of PIAS3-induced sumoylation sites in PRB. (A) A schematic representation of PRB shows the sites corresponding to consensus sequences for SUMO-1 conjugation. (B and C) 293T cells were transfected with HA-SUMO-1 and Myc-PRB, Myc-PRB K388R, Myc-PRB K7/531R, or Myc-PRB K7/388/531R in the presence or absence of PIAS3. Cells were treated, or not, with progesterone (100 nM, 2 h) before harvesting. Cell extracts were lysed with RIPA buffer and immunoprecipitated with anti-Myc antibody. The immunoprecipitates were probed with anti-Myc and anti-HA antibody, respectively.

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