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. 2006 Oct 13;34(20):5752–5763. doi: 10.1093/nar/gkl710

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© 2006 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Nicking activity on an artificial substrate. Double-stranded oligos of the sequence indicated (bottom strand sequence shown) were used as substrates. Nicks were monitored in the radiolabeled bottom strands. A 30 bp fragment from site #3 (Figure 1) was used as control for enzyme activity. An artificial sequence (S32AT) was used as substrate and positions of nicks obtained are indicated by arrows. The various mutations introduced in S32AT are shown in bold. Three extra nucleotides were added in S35AT and S35A.