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. 2006 Nov 24;34(20):5880–5891. doi: 10.1093/nar/gkl749

Figure 3.

Figure 3

The chl1 mutation confers growth sensitivity in the presence of MMS and UV rays. (A) Spot assay for MMS sensitivity of chl1::HIS3 (AP22Dchl1), AP22Δ22 (mcm22-Δ1::TRP1), and wild-type (AP22) strains. Growing cells were serially diluted and spotted on YEPD plates containing 0.025% MMS or no MMS (control). The YEPD plate was incubated at 30°C for 2 days while the MMS-containing plate was incubated at the same temperature for 3–4 days. (B) chl1 mutation confers hypersensitivity to UV rays. Exponentially growing cells, appropriately diluted, were plated on YEPD plates, exposed to UV rays for various times and incubated in the dark at 30°C. Control plates were also kept which were not irradiated and were used to calculate total number of cells plated. Colonies were counted after 2–3 days of incubation. Results are the mean values of four independent experiments and error bars are SD values from the mean.