Table 1.
Strains used in this study
| Strain | Genotype | Sources/References |
|---|---|---|
| A3 | MATaleu2-3, 112 his3-11, 15 | (24) |
| AP22 | MATα leu2-3, 112 his3-11, 15 ura3-52 trp1 | (34) |
| R67 | MATα leu2 ura3 his4 mcm12-1 | From B.-K. Tye |
| M11 | MATα leu2 ura3 his4 mcm12-2 | (24) |
| 8534-8C | MATα his4Δ34 ura3-52 leu2-3,112 | (24) |
| A3Dchl1 | MATa leu2-3, 112 his3-11, 15 chl1::HIS3 | This study |
| AP22Δ22 | MATα leu2-3, 112 his3-11, 15 ura3-52 trp1 mcm22-Δ1::TRP1 | By deleting AP22 (44) |
| AP22Dchl1 | MATα leu2-3, 112 his3-11, 15 ura3-52 trp1 chl1::HIS3 | (34) |
| M46-3C | MATα leu2-3, 112 his3-11, 15 ura3-52 mcm2-1 | (24) |
| M46-3CDchl1 | MATα leu2-3, 112 his3-11, 15 ura3-52 mcm2-1 chl1::HIS3 | This study, by disrupting CHL1 in M46-3C |
| M46-3CΔ21 | MATα leu2-3, 112 his3-11, 15 ura3-52 mcm2-1 mcm21-Δ2::LEU2 | This study |
| M46-3CDchl4 | MATα leu2-3, 112 his3-11, 15 ura3-52 mcm2-1 chl4::HIS3 | This study |
| SL13 | MATaleu2-3, 112 his3-11, 15 ura3-52 mcm2-1 | By crossing M46-3CDchl1 with A3 |
| SL13Dchl1 | MATaleu2-3, 112 his3-11, 15 ura3-52 mcm2-1 chl1::HIS3 | This study, by disrupting CHL1 in SL13 |
| 699 | MATaade2-1 trp1-1 leu2-3, 112 his 3-11, 15 ura3 can1-100 | Uttam Surana |
| 699Dchl1 | MATaade2-1 trp1-1 leu2-3, 112 his 3-11, 15 ura3 can1-100 chl1::HIS3 | This study, by disrupting CHL1 in 699 |
| SL14 | MATaade2-1 trp1-1 leu2-3, 112 his 3-11, 15 ura3 can1-100 bar1::LEU2 | This study, by disrupting BAR1 in 699 |
| SL14Dchl1 | MATaade2-1 trp1-1 leu2-3, 112 his 3-11, 15 ura3 can1-100 chl1::HIS3 bar1::LEU2 | This study, by disrupting BAR1 in 699Dchl1 |
| 699Δsgs1 | MATaade2-1 trp1-1 leu2-3, 112 his 3-11, 15 ura3 can1-100 sgs1Δ::LEU2 | This study, by deleting SGS1in 699 |
| 699Δsgs1Dchl1 | MATaade2-1 trp1-1 leu2-3, 112 his 3-11, 15 ura3 can1-100 sgs1Δ::LEU2 chl1::HIS3 | This study, by disrupting CHL1 in 699Δsgs1 |
| US456 | MATα leu2 his3 trp1 ade2 rad24::URA3 | Uttam Surana |
| SL3 | MATaleu2 his3 trp1 ade2 rad24::URA3 | By crossing US456 with 699Dchl1 |
| SL3Dchl1 | MATaleu2 his3 trp1 ade2 rad24::URA3 chl1::HIS3 | From SL3, by disrupting the CHL1gene |
| SL4 | MATaleu2 his3 trp1 ade2 rad24::URA3 chl1::HIS3 | By crossing US456 with 699Dchl1 |
| SS1 | MATaleu2 his3 trp1 ade2 rad24::URA3 bar1::LEU2 | From SL3, by disrupting the BAR1gene |
| SS1Dchl1 | MATaleu2 his3 trp1 ade2 rad24::URA3 chl1::HIS3 bar1::LEU2 | From SL3Dchl1, by disrupting the BAR1gene |
| US354 | MATα leu2 his3 trp1 ade2 ura3 rad53-21 | Uttam Surana |
| SL7 | MATaleu2 his3 trp1 ade2 ura3 rad53-21 | By crossing US354 with 699 |
| SL7Δchl1 | MATaleu2 his3 trp1 ade2 ura3 rad53-21 chl1Δ::TRP1 | This study, by deleting CHL1in SL7 |
| US3138 | MATaade2-1leu2-3,112 trp1-1 his3-11,15ura3 can1-100 bar1 GAL psi+mec1-1 | Uttam Surana |
| YB0297 | MATaade2 leu2 trp1 his3 can1 GAL psi+cdc6-3 | From B.-K. Tye |
| YB0297Dchl1 | MATaade2 leu2 trp1 his3 can1 GAL psi+cdc6-3 chl1::HIS3 | This study, by disrupting CHL1 in YB0297 |
| JRY4490 | MATacan1-100 his3-11 leu2-3, 112 lys2Δ trp1-1 ura3-1 orc2-1 | From B.-K. Tye |
| JRY4245 | MATaade2-1 can1-100 his3-11 leu2-3, 112 trp1-1 ura3-1 orc5-1 | From B.-K. Tye |
| JRY4245Dchl1 | MATaade2-1 can1-100 his3-11 leu2-3, 112 trp1-1 ura3-1 orc5-1 chl1::HIS3 | This study, by disrupting CHL1 in JRY4245 |