Table 2.
chl1enhances the cell cycle arrest phenotype of mcm2-1cells
| Strain | Hours at 32°C | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 | 4 | 8 | 24 | |||||
| % Large-budded | % Arrested | % Large-budded | % Arrested | % Large-budded | % Arrested | % Large-budded | % Arrested | |
| (MCM2 CHL1) | 11 | 0 | 7.4 | 0.7 | 3 | 0 | 4.7 | 0.8 |
| (MCM2 chl1) | 27 | 7 | 15 | 6.4 | 17 | 9.2 | 2.3 | 0.6 |
| (mcm2-1 CHL1) | 17 | 1 | 42 | 23 | 35 | 16 | 27 | 10 |
| (mcm2-1 chl1::HIS3) | 30 | 15 | 65 | 43 | 77 | 47 | 60 | 26 |
Cells from Figure 2 were analyzed for cell cycle arrest (percent large-budded cells in the culture) and nuclear morphology (percent large-budded cells in the culture which had single nucleus at the neck or stretched through it). About 150–200 cells were analyzed in each case.