Figure 13.

Stimulation of the L11–RNA interaction by L10/L12₄, analyzed by non-denaturing PAGE with two color fluorescent staining. The protein component was pre-labeled (i.e. before complex formation) with TAMRA (red) and the gel was stained with SYBR^® Green I, specific for RNA (green). The yellow color represents the protein–RNA complexes because of the superimposed green and red signals from the RNA (SYBR Green I) and protein (TAMRA) channel. Lane 1, RNA fragment Mja23S-95; lanes2 and 3, pre-labeled MjaL11 mixed with the RNA fragment in a molar molar ratio of 1:2, incubated at 37 and 70°C, respectively; 4, pre-labeled MjaL11; lanes 5 and 6, mixture of MigL10/L12₄–RNA–pre-labeled MjaL11 in a molar ratio of 1:2:1, incubated at 37 and 70°C, respectively; lanes 7 and 8, pre-labeled MigL10/L12₄ mixed with RNA fragment in a molar ratio of 1:2, incubated at 37 and 70°C, respectively; 9, pre-labeled MigL10/L12₄. RNA, RNA fragment Mja23S-95; PC-R, L10/L12₄ bound to RNA; L11-R, L11 bound to RNA; L11-PC-R, complex of L11 and L10/L12₄ with RNA.