Skip to main content
. 2006 Oct 19;34(20):5800–5814. doi: 10.1093/nar/gkl751

Figure 2.

Figure 2

Interaction of archaeal L10/L124 complexes with the specific Mja23S-95 rRNA fragment analyzed by non-denaturing PAGE with two color fluorescent staining. The protein component is visualized with SYPRO® Red Protein Gel Stain (red) and the RNA component with SYBR® Green I (green). The yellow color represents the protein–RNA complexes because of the superimposed green and red signals from the RNA (SYBR Green I) and protein (SYPRO Red) channel. Lane 1, RNA fragment Mja23S-95; lanes 2–4, MigL10/L124 mixed with Mja23S-95 in a molar ratio of 1:2, 1:1, 2:1 respectively; lanes 5–7, MthL10/L124 mixed with Mja23S-95 in the same ratios; 8, 9, 10—MvaL10/L124 mixed with Mja23S-95 in a molar ratio of 1:4, 1:1, 4:1, respectively. R, RNA fragment Mja23S-95; mP(R), monomeric form of the L10/L124 (bound to RNA); dP(R),dimeric form of the L10/L124 (bound to RNA); trP,trimeric form of the L10/L124; teP, tetrameric form of the L10/L124.