Table 2.

23S rRNA fragments used for filter-binding assays and gel-shift experiments

Source organism	Name	Template used for transcription	Size of transcript, nt	Short description
M.jannaschii	pMja23S-95	Plasmid	95	Full-length binding site (boxed green in Figure 6B)
	Mja23S-80	PCR fragment	80	Binding site where helix 43a was deleted
	Mja23S-70	––//––	70	Binding site where whole helix 43 was deleted
	Mja23S-81	––//––	81	Binding site where helix 44 was deleted
	Mja23S-56	––//––	56	Binding site where both of helixes 43 and 44 were deleted
M.thermolithotrophicus	pMth23S-95	Plasmid	95	Full-length binding site (designed as Mja23S-95)
M.vannielii	pMvL10.23S	––//––	230	Complete L10 binding site in 23S rRNA from M.vannielii (26)
S.solfataricus	pSso23S-95	––//––	95	Full-length binding site (designed as Mja23S-95)
T.thermophilus	Tth23S-95	PCR fragment	95	––//––
G.stearothermophilus	Gst23S-95	––//––	95	––//––
E.coli	Eco23S-95	––//––	95	––//––