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. 2006 Oct 24;34(20):5892–5905. doi: 10.1093/nar/gkl752

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© 2006 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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HPLC chromatograms of tRNA hydrolysates. Strains used were: MG1655 (wild-type), IC5241 (gidA::Tn10), and IC5348 (mnmE::kan), in (A), and IC5241 transformed with plasmids pIC1154, pIC1177, pIC1178 and pIC1179 (carrying the wild-type, G13A, G15A and G13A/G15A gidA allele, respectively), in (B). Strains in (B) were grown with (+, right side) or without (−, left side) IPTG. The nucleosides were monitored at 314 nm to maximize the detection of thiolated nucleosides. mnm⁵s²U and s²U were identified by comparing UV spectra with published spectra (41). AU, absorbance units.