. 2006 Oct 27;34(20):6034–6043. doi: 10.1093/nar/gkl765

Table 1.

Efficiency of C₃₄ to m⁵C₃₄ conversion in HeLa extract

Mutant name	Mutation site	Relative amount of m⁵C₃₄ (%)
pHLIVS2	wt	100
Mini-helix	pre-tRNA^Leu anticodon stem-containing intron	0 (undetectable)
pHLIVS2 Δ I	Intron deleted	0
pHLIVS2 Δ I 1	Intron arm I deleted	0
pHLIVS2 ΔI 2	Intron arm II deleted	0
pHLIVS2 A₃₂	C₃₂ → A₃₂	85
pHLIVS2 G₃₂	C₃₂ → G₃₂	0
pHLIVS2 U₃₂	C₃₂ → U₃₂	85
pHLIVS2 A₃₃	U₃₃ → A₃₃	85
pHLIVS2 C₃₃	U₃₃ → C₃₃	10
pHLIVS2 G₃₃	U₃₃ → G₃₃	10
pHLIVS2 C₃₅	A₃₅ → C₃₅	0
pHLIVS2 G₃₅	A₃₅ → G₃₅	0
pHLIVS2 U₃₅	A₃₅ → U₃₅	0
pHLIVS2 C₃₆	A₃₆ → C₃₆	0
pHLIVS2 G₃₆	A₃₆ → G₃₆	0
pHLIVS2 U₃₆	A₃₆ → U₃₆	0
pHLIVS2 A₃₇	G₃₇ → A₃₇	0
pHLIVS2 C₃₇	G₃₇ → C₃₇	0
pHLIVS2 U₃₅A_11i	A₃₅ → U₃₅, U_11i → A_11i	0
pHLIVS2 U₃₇	G₃₇ → U₃₇	0
pHLIVS2 G₃₆C_i10	A₃₆ → G₃₆, U_i10 → C_i10	85
pHLIVS2 C₃₇G_i9	G₃₇ → C₃₇, C_i9 → G_i9	0
pHLIVS2 G_i9A_i10A_i11	C_i9 → G_i9, U_i10 → A_i10, U_i11 → A_i11	0

³²P-labelled pre-tRNAs^Leu were incubated in cell-free nuclear extract for 20 min at 30°C. The intron-containing pre-tRNA^Leu and its mutants were analysed for the presence of m⁵C₃₄ after T₂ digestion by thin layer chromatography as described in Materials and Methods. The efficiency of m⁵C₃₄ formation was calculated as described in Materials and Methods.