Figure 6.

WIPa translocates to the cell cortex upon global cAMP stimulation. (A) Images of cells expressing YFP-WT WIPa or K36A mutant globally stimulated with 100 μM cAMP are shown. Data points were taken at 3-s intervals. Two linescan measurements from at least 24 cells of each cell type were used for quantitation of YFP intensity at the membrane. (B) F-actin pulldown of YFP-WIPa. WIPa interaction with F-actin depends on WH2 domain and PI3 kinase activity. (C) In vivo F-actin polymerization assay. WT WIPa overexpression leads to an increase in actin polymerization 30 s after global cAMP stimulation.