Figure 8.

VASP is required for WIPa-induced microspike formation and membrane translocation. (A) Overexpression of WIPa fails to significantly increase the steady state level of microspikes in a vasp null background. The number of microspikes from at least 50 cells was counted in each of three independent experiments. (B) YFP-WIPa translocation. Significantly less WIPa translocates to the cell cortex upon cAMP stimulation in the absence of VASP. (C) F-actin pulldown of YFP-WIPa is decreased in VASP null cells. (D) In vivo F-actin polymerization in response to cAMP is defective in VASP-null cells. (E) Cortical translocation of the F-actin marker GFP-ABP in response to global cAMP stimulation is defective in VASP null cells.