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GCC185 depletion leads to minimal disruption of Golgin-245 localization and Golgi stacking. GCC185 (top left) and Golgin-245 (top right) in control, mock transfected or GCC185-depleted cells. A single cell in the second row, top right quadrant, was not depleted of GCC185; three other cells in the field were depleted and labeled for Golgin-245 (second row, right). Bar, 20 μm. Electron microscopy of control or GCC185-depleted HeLa cells (bottom). Bar, 500 nm.
