Figure 5.

Dispersal of CI-MPR vesicles in cells depleted of GCC185. Left, perinuclear localization of CI-MPR in control HeLa cells. Right, dispersed localization of CI-MPR in cells of similar size, depleted of GCC185. The images were acquired on a deconvolution microscope and a total summation of the Z-sections is shown. Approximate cell outlines are drawn in white. The fluorescence intensity of CI-MPR in 10 GCC185-depleted and five control cells was quantified using ImageJ. Circles of different radii, centered around the peak fluorescent signal were drawn, and the intensity within the circle was quantified relative to the total intensity in the whole cell; data are presented in relation to the concentric circle pattern shown. Bar, 5 μm.