Figure 9.

TIP-1 expression reduces amount of Kir 2.3 associated with the mLin-7/CASK complex. (A) Confluent MDCK Kir 2.3-VSV cells were infected with wild-type (WT) or H90A TIP-1-myc adenoviruses and compared with mock-infected cells (Ad/TIP-1-myc−). Lysates were immunoprecipitated with anti-mLin-7c antibodies (+) or no-antibody (−) and then immunoblotted with anti-VSV antibodies for Kir 2.3-VSV (above) or anti-CASK antibodies (below). (B) Mean ± SE densitometry (immunoprecipitated protein/input) from three separate infections; n = 3; p ≤ 0.05.