Figure 1.
MAP1B expression is up-regulated upon induced differentiation of the CG4 oligodendrocyte cell line. (A) Morphogenesis of CG4 cells during induced differentiation. Days for differentiation are marked on each panel. (B) A representative phosphorimage showing increased MBP mRNA expression as a marker of oligodendrocyte maturation during CG4 cell differentiation. Total RNA isolated from CG4 cells that had undergone various days of differentiation as indicated on the top of the corresponding lanes was subjected to a semiquantitative RT-PCR analysis using 32P-labled primers specific for MBP. RT-PCR of the housekeeping GAPDH mRNA was performed in parallel as an input reference. (C) Immunoblot analysis detects marked up-regulation of MAP1B protein upon induced differentiation of CG4 cells. The blot was reprobed with the housekeeping protein eIF5a to provide a loading control. (D) a–c, localization of MAP1B (green) and MBP (red) in differentiating CG4 cells; d–f, localization of MAP1B (green) and α-tubulin (red) in differentiating CG4 cells.