Figure 2.

Elevated MAP1B mRNA expression during CG4 cell differentiation. (A) Representative RPA gel showing MAP1B mRNA levels in CG4 cells that had undergone various days of differentiation as indicated on the top of the corresponding lanes. RPA of β-actin mRNA was performed in the same reaction to provide a loading control. (B) Real-time RT-PCR (qRT-PCR) quantification of the MAP1B mRNA levels during CG4 cell differentiation. Total RNA was extracted from four independent sets of differentiated CG4 cells (n = 4). The qRT-PCR reading of MAP1B mRNA in each sample was normalized to that of the housekeeping mRNA GAPDH. The normalized MAP1B mRNA level in undifferentiated cells (D0) was set as 100% in each set of sample. p < 0.01 based on one-way ANOVA.