Figure 2.

Dose dependence of blebbistatin effects on cytoskeletal asymmetry. The asymmetric distributions of F-actin, myosin IIA, and myosin IIB 1 h after wounding an endothelial monolayer were measured by determining their displacements relative to the center of mass of protein in the cell as described in Materials and Methods. The magnitude of the asymmetry indicates the displacement of the center of mass toward the wound, with a negative asymmetry indicating accumulation away from the wound (i.e., toward the rear of the cell). Each point represents the mean of measurements from 20 to 40 randomly selected cells; error bars, 1 SD.