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. 2006 Oct;17(10):4576–4583. doi: 10.1091/mbc.E06-03-0232

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© 2006 by The American Society for Cell Biology

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Figure 2. — Light induces a transient increase in acetylation of histone H3. (A) Acetylated histones accumulate 20 min after a light pulse. Left, representative PCR coamplifications of the al-3 LRR and the actin promoter (internal control) from chromatin immunoprecipitations of Neurospora WT (FGSC987) with antibodies directed against acH3 IP or H3 IP. INPUT represents the sample before immunoprecipitation. Right, histograms derived from three independent amplifications with two independent immunoprecipitations. (B) H3 K14 is the target for transient acetylation after a light pulse. Left, representative PCR coamplifications of the al-3 and actin promoter regions 20 min after a light pulse (see A) after immunoprecipitation with antibodies directed against acH3 IP or H3 acetylated specifically on K14 (acK14H3 IP) Right, histograms derived from three independent amplifications with two independent immunoprecipitations.