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Redistribution of Golgi to the distal region of hyphae does not require microtubules. Cultures of wild-type cells expressing either CaVRG4-GFP, CaTUB1-GFP, or CaTUB2-GFP were induced to form hyphae in the presence (+NZ) or absence (−NZ) of 5 μM NZ. Samples were harvested after 1.5 h of hyphal induction and stained with DAPI for the visualization of CaVrg4-GFP, Tub1-GFP and Tub2-GFP or nuclei, as described in Materials and Methods. Bars, 5 μm.
