Figure 4.

Maintenance of Golgi organization near the hyphal tip requires an intact actin cytoskeleton. (A) Actin inhibition results in the disorganization of Golgi in hyphal cells. Stationary phase cultures of wild-type cells harboring CaVRG4-GFP, MLC1-YFP, or ABP1-YFP were induced to form hyphae for 1 h 45 min. Cells were treated with buffer alone (DMSO + SDS) (−CA) or buffer containing 20 μg/ml CA (+CA) for 45 min before visualization of cells by fluorescence microscopy. (B) CA inhibits actin organization in hyphal cells. Cells grown as described in A were incubated in the presence or absence of CA, fixed, and stained with Alexa 546-phalloidin as described in Materials and Methods. (C) Treatment of hyphal cells with CA has no effect on ER organization. BWP17 cells expressing ER-GFP were induced to form hyphae and then incubated in the presence or absence of 20 μg/ml CA for 45 min as described in A and visualized by fluorescence microscopy. Bars, 5 μm.