Table 3.

NZ (5 μM) depolymerizes microtubules and inhibits nuclear division in C. albicans

Cells	% Cells with at least one visible microtubule	% Cells with divided nuclei	% Large-budded cells
(−) Nocodazole	100.0 (n = 384)	27.3 (n = 300)	51.7 (n = 300)
(+) Nocodazole	3.3 (n = 272)	2.7 (n = 289)	97.0 (n = 300)

Cells expressing Tub2-GFP were grown overnight into stationary phase in YPD, diluted to an OD₆₀₀ of 0.3, and allowed to grow in the presence or absence of 5 μM nocodazole at 30°C for 2 h 15 min before harvesting. Cells were washed once in PBS, and microtubules were visualized and quantitated directly by fluorescence microscopy. Large-budded cells were cells where the daughter cell was at least two-thirds the size of the mother cell. The status of nuclear division was assessed by staining cells with DAPI.