Figure 9.

Karyotype determination in Clasp2 KO adult fibroblasts. (A–A′) Chromosome spread from a WT adult fibroblast showing DNA (blue) and ACA staining at kinetochores (red). The normal euploid number of chromosomes in mice is 40. (B–B′) Chromosome spread from a Clasp2 KO adult fibroblast showing DNA (blue) and ACA staining at kinetochores (red). A higher chromosome number is evident. (C) Quantification of the typical chromosome number in WT and Clasp2 KO MAFs. Clasp2 KO MAFs show severe chromosomal instability with a peak distribution between 71 and 75 chromosomes per cell. Bar, 10 μm.