Figure 4.

Deoxyglucose transport. Glucose uptake in oocyte cells overexpressing Arabidopsis SGB1 protein. The indicated RNA (50 ng) was injected into oocyte cells. Glucose uptake was carried out on the third day as described in Materials and Methods. Two negative controls were antisense SGB1 RNA and a frame-shifted mutant RNA of SGB1, which introduces a premature stop codon. The positive control was a constitutively active human glucose transporter designated GLUTX1′LL-AA. The inset shows the immunoblot analysis of oocyte extracts probed with serum directed against SGB1 as described in Materials and Methods.