Figure 8.

Recruitment of CVAK104 and AP1 by permeabilized cells. PtK cells were permeabilized by treatment with 40 μg/ml digitonin for 5 min on ice. The cells were then incubated at 30°C for 30 min with rat brain cytosol either alone (A) or in the presence of an ATP-regenerating system (B), GTPγS (C), or in the presence of both an ATP-regenerating system and GTPγS (D). Cells were fixed and immunostained for AP1 and CVAK104. Recruitment of CVAK104 only occurs in the presence of GTPγS, indicating that it requires a GTP binding protein. Bars, 10 μm.