Figure 1.

Summary of the functional characteristic of Mlph constructs used in this study. The top part of the figure is a schematic representation of the domain structure of Mlph showing the regions previously shown to be involved in interaction with other proteins. In the bottom part of the figure solid lines indicate wild-type Mlph sequence, dashed lines indicate internal truncations, gray shaded areas indicate non-Mlph sequence within chimeric proteins, and letters A and X indicate the position of ala or other substitutions in point mutants. Each construct is scored + or − for its ability to be recruited to melanosomes, the ability to recruit MyoVa to melanosome and its ability to rescue melanosome transport defects in melan-ln cells.