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. 2006 Nov;17(11):4720–4735. doi: 10.1091/mbc.E06-05-0457

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© 2006 by The American Society for Cell Biology

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Figure 5. — Rescue of melan-ln melanosome transport defects requires part of the ABD in addition to R27BD and MBD sequences. Melan-ln melanocytes were transfected with plasmids encoding the indicated C-terminus–truncated Mlph molecules, fixed 48 h later, and stained with antibodies to detect myc-tagged Mlph and MyoVa. (A, E, I, M, Q, and U) The distribution of myc-tagged Mlph molecule as indicated. (B, F, J, N, R, and V) The distribution of endogenous MyoVa. (C, G, K, O, S, and W) Merged fluorescence images showing the overlap of myc-tagged Mlph (green) and MyoVa (red). (D, H, L, P, T, and X) The corresponding transmission images showing the distribution of pigment in transfected cells. Arrows indicate colocalization of overexpressed protein with pigmented melanosomes. Bar, 20 μm.