Figure 8.

Frap analysis of actin-GFP. (a) Images of control (elav^3A-Gal4 × Actin-GFP) and NSF2^E/Q (elav^3A-Gal4::UAS-NSF2^E/Q × UAS-Actin-GFP) nerve terminals expressing actin-GFP subjected to FRAP. Arrow indicates the region that was photobleached, 0 indicates before photobleaching, 0.5 is immediately after photobleaching, and 10 and 20 are 10 and 20 s after photobleaching. Scale bar, 5 μm. (b) The time course of recovery of actin-GFP signal after photobleaching, where the symbols represent the mean value and the bars the SEM at each time point. The line is the best fit of the one-phase exponential equation y = A(1 − e^(−k/x)) + B to the recovery data points. Scale bar, 2 μm. (c) Summary of the % recovery values obtained for control and NSF2^E/Q boutons, in the absence or presence of 5 μM swinholide. Sample size (n = number of boutons) is indicated on each bar, from three larvae each.