Figure 5.

Translocation of GFP-Prz1 to the nucleus upon treatment with DTT or micafungin. The Δprz1 cells transformed with the vector containing GFP-Prz1 were grown in EMM medium containing thiamine at 30°C. Cells treated with 0.5 M NaCl, 3.2 mM DTT, or 4 μg/ml micafungin for 1, 3, or 2 h, respectively, were observed under the fluorescence microscope. Bar, 10 μm.