Figure 8.

Overexpression of constitutively active Pek1 MAP kinase kinase induces Ca²⁺ influx. Wild-type, Δyam8, Δpmk1, or Δpck2 cells were prepared and cultured as in Figure 7A. Appropriate treatments were added as indicated then supplemented with tracer quantities of ⁴⁵Ca²⁺, and incubated for 4 h at 30°C. The data were averaged from three independent experiments, and each sample was done in duplicate. Bars, SD.