Table 5.
The 3xCDRE::luc(R2.2) reporter activity in various knockout cells
| Cell types |
Stimuli | |||
|---|---|---|---|---|
| CaCl2 (100 mM) |
NaCl (0.5 M) |
Micafungin (4 μg/ml) |
Chlorpromazine (250 μg/ml) |
|
| Δyam8 | 102 ± 11 | 2.3 ± 0.4 | 2.5 ± 0.3 | 103 ± 11 |
| Δcch1 | 98 ± 13 | 3.3 ± 0.5 | 3.1 ± 0.3 | 99 ± 7 |
| Δpmk1 | 95 ± 12 | 18.6 ± 1.5 | 21.3 ± 2.2 | 93± 14 |
| Δpek1 | 101 ± 15 | 22.3 ± 2.3 | 21.3 ± 1.8 | 104 ± 12 |
| Δmkh1 | 105 ± 9 | 19.7 ± 1.6 | 20.1 ± 2.4 | 108 ± 9 |
| Δpck2 | 85 ± 18 | 13.3 ± 1.4 | 15.3 ± 2.8 | 78 ± 15 |
Various knockout cells were transformed with the integration plasmid containing the 3xCDRE::luc(R2.2) reporter gene. Cells were stimulated by various treatments as indicated and the calcineurin activity in living cells was monitored. In each case, the peak height of each response was compared with that of the wild-type cells. Mean ± SD values from at least three independent experiments are expressed as the percentage of wild-type cells.