Figure 7.

The BAX, BAK ER gateway controls death by hFis1. (A) hFis- induced apoptosis is restored in DKO-SERCA but not in DKO-mtBAX MEFs. MEFs of the indicated genotype were cotransfected with GFP and the indicated plasmids. Apoptosis was determined as the percentage of GFP-positive, Annexin-V–positive cells by flow cytometry. Data represent mean ± SE of six different experiments. (B) hFis1 induced cell death is inhibited by low extracellular [Ca²⁺] and by NAC. wt MEFs were transfected as in A, and after 4 h NAC (2.5 mM) was added to the media. Apoptosis was monitored 48 h after transfection. Where indicated (0.1 mM Ca²⁺), wt MEFs were incubated in KRB supplemented with EGTA for 3 h and then maintained in complete DMEM containing 0.1 mM Ca² before transfection.