Figure 2.

Src phosphorylates RhoGDI at Tyr156 in vivo. HeLa cells were cotransfected with active Src Y527F (or empty vector) and the indicated mutant versions of His-tagged RhoGDI. The top panel shows equal amounts of each construct were immunoprecipitated. Immunoblot of immunoprecipitated RhoGDI constructs with 4G10 phosphotyrosine antibody indicate that RhoGDI is primarily phosphorylated by Src at Tyrosine 156.