Table 2.

HMGCR gene expression changes in human astrocytoma and neuroblastoma cell lines

	Haloperidol		Clozapine
Cell type	10 μM	25 μM	10 μM	25 μM
SH-SY5Y	1.3 ± 0.06*	1.5 ± 0.1*	0.9 ± 0.02	1.4 ± 0.1*
CCF-STTG1	5.3 ± 0.6**	9.3 ± 0.4***	1.5 ± 0.2*	5.0 ± 0.2***
HCN2	1.2 ± 0.03*	ND	ND	2.4 ± 0.2 **

Human CCF-STTG1 astrocytoma and SH-SY5Y neuroblastoma cells were exposed to haloperidol and clozapine (10 μM and 25 μM of each drug), and human cortical neuron HCN2 cells were exposed to haloperidol (10 μM) and clozapine (25 μM). The HMGCR-expression was determined relative to the expression of P0 in all cell lines. For CFF-STTG1 and SH-SY5Y, statistical significance of drug-induced HMGCR expression was determined by the Dunnetts post-hoc test that correct for multiple testing, as described in Materials and methods. For the HCN2-cells that had only two comparisons, the student's t-test was applied. Each statistical comparison was performed between drug treated cells and vehicle treated control. Significance was denoted as follows: *p < 0.05, **p < 0.01, ***p < 0.001. The fold changes (mean ± SEM) are representative of three independent parallel experiments and measured relative to vehicle treated control. ND: Not determined.