Figure 4. (A) 600 MHz one-dimensional, (B) TOCSY and (C) NOESY NMR spectra for cycloviolacin O14.

All spectra were recorded at 290 K in 90% water/10% ²H₂O at pH 3.5. (B) Amino acid spin systems are labelled. Note the absence of signals for residues Phe¹¹ and Lys¹² and the broadened signals for Cys¹⁰ and Gly¹³ (arrowed in the one-dimensional spectrum). The peaks for Tyr²⁵ are boxed, as there were no observable TOCSY signals for this residue. The ‘fingerprint’ region of the NOESY spectrum (C) shows the sequential connectivities between the residues.