Figure 7. Cyclotides from the (A) bracelet and (B) Möbius subfamilies from V. odorata.

The bracelet and Möbius subfamilies are represented by cycloviolacin O1 and O14 respectively and their backbone sequences are indicated in the inner ‘backbone wheel’. Empty circles represent points where insertions are found in other cyclotides compared and the variations for each position are given by additional amino acids outside the ‘backbone wheel’. The more abundant an amino acid is at a particular position, the closer it is to the representative backbone. Disulfide bonds and loops are represented and the protein precursor processing point is also indicated (heavy arrow in A). The cis-proline bond that defines the Möbius subfamily is also indicated.