Figure 3. IL-1β induces p65 binding to the IκBβ gene promoter in vivo.

1321N1 astrocytoma were treated with or without IL-1β (10 ng/ml) for 1 h. Nuclear lysates were generated and immunoprecipitated with anti-p65 or control IgG antibodies. Immunoprecipitated DNA was analysed for IκBα, IκBβ, IL-2 and GAPDH promoters by PCR and gel electrophoresis (A) and IκBα, IκBβ and IL-2 promoters by quantitative real-time PCR (B). Data from real-time PCR are expressed as fold differences between DNA enrichment in the anti-p65 ChIP sample relative to IgG ChIP sample and are displayed as means±S.E.M. for three to five independent experiments. *P<0.05, **P<0.01 versus unstimulated control (unpaired t test).